Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials · 2016
[Study on Variation of Gastroprotective Effect of Atractylodes lancea Rhizome Before and After Processing].
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Yu Yan, Cai Qian, Jia Tian-zhu
Аннотация
OBJECTIVE: To compare the gastroprotective effect between the crude and bran-processed Atractylodes lancea rhizome in the rat model of gastric ulcer.
METHODS: The model of gastric ulcer was imitated by acetic acid in rats. The levels of epidermal growth factor( EGF) and trefoil factor 2( TFF2) in serum and stomach were determined by the double-antibody sandwich enzyme-linked immunosorbent assay( ELISA),and the mRNA expressions of EGF and TFF2 in stomach were analyzed by quantitative real-time reverse transcription polymerase chain reaction( RT-PCR). The protein expressions of EGF and TFF2 in stomach were examined by immunohistochemistry.
RESULTS: The productions of EGF,TFF2 and the mRNA expressions of EGF and TFF2 were notably up-regulated by both crude Atractylodes lancea rhizome and bran-processed Atractylodes lancea rhizome. Compared with the crude Atractylodes lancea rhizome,the processed Atractylodes lancea rhizome was more effective.
CONCLUSION: The gastroprotective effect of Atractylodes lancea rhizome was enhanced after stir-frying with wheat bran in the rat model of gastric ulcer induced by acetic acid.
METHODS: The model of gastric ulcer was imitated by acetic acid in rats. The levels of epidermal growth factor( EGF) and trefoil factor 2( TFF2) in serum and stomach were determined by the double-antibody sandwich enzyme-linked immunosorbent assay( ELISA),and the mRNA expressions of EGF and TFF2 in stomach were analyzed by quantitative real-time reverse transcription polymerase chain reaction( RT-PCR). The protein expressions of EGF and TFF2 in stomach were examined by immunohistochemistry.
RESULTS: The productions of EGF,TFF2 and the mRNA expressions of EGF and TFF2 were notably up-regulated by both crude Atractylodes lancea rhizome and bran-processed Atractylodes lancea rhizome. Compared with the crude Atractylodes lancea rhizome,the processed Atractylodes lancea rhizome was more effective.
CONCLUSION: The gastroprotective effect of Atractylodes lancea rhizome was enhanced after stir-frying with wheat bran in the rat model of gastric ulcer induced by acetic acid.