Sensitive miRNA Detection for Early Diagnosis of Psoriasis Based on Dual Signal Recycles.

MicroRNAs (miRNAs) play a pivotal role in regulating a variety of biological processes and can be used as biomarkers for the early diagnosis of various diseases, such as psoriasis. Herein, we depict a simple and sensitive miRNA detection method based on dual signal recycles, which is developed on the basis of strand displacement amplification (SDA). The sensor is successfully applied to the detection of miRNA-21 with a wide linear range from 100 fM to 10 nM and a lower limit of detection (LOD) of 67 fM. Because of the simple operation yet improved detection capability, we thereby believe that the developed fluorescent biosensor can be potentially applied for early clinical diagnosis as well as biological researches.