Recent advancements in liquid chromatography column technologies: Manufacturing, connecting and parallel using.

The evolution of omics science and pharmaceutical research has driven the fast development of high-throughput liquid chromatography (HTLC). Among the various technologies enabling HTLC, column technology plays a crucial role - it directly increases analytical throughput while also securing the resolution of HTLC systems. This review summarizes recent key advancements in liquid chromatography column technologies for HTLC, focusing on three major aspects: column manufacturing, connecting, and parallel using. The integration and development of manufacturing, connecting and parallel using of chromatographic columns provide the underpinning support for the fields demanding high analytical throughput, such as single-cell proteomics, drug discovery, and large-scale screening analyses.