Clinical laboratory · 2011
Lipid peroxidation and parathyroid hormone influence the cytosolic calcium levels of erythrocytes in peritoneal dialysis patients.
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Sahin Emel, Goçmen Ayşe Yeşim, Gümüşlü Saadet, Sahin Mehmet, Koçak Hüseyin, Tuncer Murat
Аннотация
BACKGROUND: The aim of this study was to examine the alterations in calcium and lipid peroxidation in red blood cells (RBCs) and serum samples of continuous ambulatory peritoneal dialysis (CAPD) patients. We also investigated the relationship between parathyroid hormone (PTH) and calcium homeostasis in this study.
METHODS: For this purpose, routine blood counts and blood chemistry were analyzed by standard laboratory procedures in serum samples. The concentration of TBARS was measured in erythrocytes and serum samples. RBC calcium was measured by Fura-2AM in a spectrofluorometer.
RESULTS: In CAPD patients, hemoglobin, albumin, and high density lipoprotein cholesterol levels were lower, but glucose, very low density lipoprotein cholesterol, triglyceride, magnesium, PTH, sensitive C-reactive protein, and uric acid levels were higher than the controls. Thiobarbituric acid-reactive substance (TBARS) levels in RBCs and serum samples and cytosolic calcium in RBCs were all found to be significantly increased in CAPD patients compared to control subjects. Multiple regression analysis showed that RBC TBARS and serum PTH were the independent predictors of RBC calcium in our study.
CONCLUSIONS: Our results confirm that oxidative stress is an important risk factor for CAPD. The results of multiple regression analysis suggest that RBC calcium was affected by both increased levels of TBARS and PTH.
METHODS: For this purpose, routine blood counts and blood chemistry were analyzed by standard laboratory procedures in serum samples. The concentration of TBARS was measured in erythrocytes and serum samples. RBC calcium was measured by Fura-2AM in a spectrofluorometer.
RESULTS: In CAPD patients, hemoglobin, albumin, and high density lipoprotein cholesterol levels were lower, but glucose, very low density lipoprotein cholesterol, triglyceride, magnesium, PTH, sensitive C-reactive protein, and uric acid levels were higher than the controls. Thiobarbituric acid-reactive substance (TBARS) levels in RBCs and serum samples and cytosolic calcium in RBCs were all found to be significantly increased in CAPD patients compared to control subjects. Multiple regression analysis showed that RBC TBARS and serum PTH were the independent predictors of RBC calcium in our study.
CONCLUSIONS: Our results confirm that oxidative stress is an important risk factor for CAPD. The results of multiple regression analysis suggest that RBC calcium was affected by both increased levels of TBARS and PTH.
Предметные рубрики MeSH
AdultBlood Cell CountC-Reactive ProteinCalciumCase-Control StudiesCytosolErythrocytesFemaleHemoglobinsHumansHyperparathyroidism, SecondaryKidney Failure, ChronicLipid PeroxidationLipidsMagnesiumMaleOxidative StressParathyroid HormonePeritoneal Dialysis, Continuous AmbulatorySerum AlbuminThiobarbituric Acid Reactive SubstancesUric Acid