Function (Oxford, England) · 2021
Mitochondrial P2X7 Receptor Localization Modulates Energy Metabolism Enhancing Physical Performance.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Sarti Alba Clara, Vultaggio-Poma Valentina, Falzoni Simonetta, Missiroli Sonia, Giuliani Anna Lisa, Boldrini Paola, Bonora Massimo, Faita Francesco, Di Lascio Nicole, Kusmic Claudia, Solini Anna, Novello Salvatore, Morari Michele, Rossato Marco, Wieckowski Mariusz R, Giorgi Carlotta, Pinton Paolo, Di Virgilio Francesco
Аннотация
Basal expression of the P2X7 receptor (P2X7R) improves mitochondrial metabolism, Adenosine 5'-triphosphate (ATP) synthesis, and overall fitness of immune and non-immune cells. We investigated P2X7R contribution to energy metabolism and subcellular localization in fibroblasts (mouse embryo fibroblasts and HEK293 human fibroblasts), mouse microglia (primary brain microglia, and the N13 microglia cell line), and heart tissue. The P2X7R localizes to mitochondria, and its lack (1) decreases basal respiratory rate, ATP-coupled respiration, maximal uncoupled respiration, resting mitochondrial potential, mitochondrial matrix Ca2+ level, (2) modifies expression pattern of oxidative phosphorylation enzymes, and (3) severely affects cardiac performance. Hearts from P2rx7-deleted versus wild-type mice are larger, heart mitochondria smaller, and stroke volume, ejection fraction, fractional shortening, and cardiac output, are significantly decreased. Accordingly, the physical fitness of P2X7R-null mice is severely reduced. Thus, the P2X7R is a key modulator of mitochondrial energy metabolism and a determinant of physical fitness.