Journal of dentistry · 2025
Development of a novel silver complex fluoride for caries management: an in vitro study.
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Xu Grace Yuchan, Yin Iris Xiaoxue, Zhao Irene Shuping, Lung Christie Ying Kei, Lo Edward Chin Man, Chu Chun Hung
Аннотация
OBJECTIVE: The objective of this study is to develop a novel antibacterial and non-discolouring silver complex fluoride (SCF) for caries management.
METHODS: SCF was synthesized by reacting 1,3,5-triaza-7-phosphaadamantane (PTA) with silver fluoride and characterised by electrospray ionization mass spectrometry. Silver and fluoride concentrations were measured by inductively coupled plasma atomic emission spectroscopy and fluoride ion-specific electrode. Alkalinity was measured using a pH meter. Cytotoxicity was assessed by determining the half maximal inhibitory concentration (IC50) against the human gingival fibroblasts (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Demineralised human dentine blocks were treated with SCF, incubated for 7 days with Streptococcus mutans, and assessed for antibacterial activity and discolouration. Biofilm kinetics and viability were analysed via colony-forming unit (CFU) counts and confocal laser scanning microscopy (CLSM). Dentine discolouration was quantified using colour spectrophotometry. Controls included 38% silver diamine fluoride (SDF; positive) and water (negative).
RESULTS: At 180 days, 55% SCF solution contained 127,498 ± 3,535 ppm silver and 27,215 ± 118 ppm fluoride, with a pH of 10. SCF demonstrated higher IC50 values compared to SDF in HGF-1 (35.5 ± 1.1 ppm vs. 17.7 ± 0.5 ppm; p < 0.001) and SHED (95.5 ± 0.1 ppm vs. 6.4 ± 0.3 ppm; p < 0.001). Log CFU counts for the SCF, SDF, and Water groups were 4.2 ± 0.2, 3.8 ± 0.4 and 5.2 ± 0.2 (SCF, SDF<Water, p < 0.001). CLSM showed dead-to-live ratios of SCF, SDF and Water group were 1.0 ± 0.3, 1.4 ± 0.5, and 0.2 ± 0.1 (p < 0.001; SCF, SDF > Water). Spectrophotometric analysis revealed ΔE values of SCF, SDF and Water groups were 5.8 ± 3.3, 73.0 ± 17.6, and 5.3 ± 2.7 (p < 0.001; SCF, Water < SDF).
CONCLUSION: A novel SCF solution with potent antibacterial efficacy and minimal discolouration was successfully developed.
CLINICAL SIGNIFICANCE: SCF demonstrates promising potential for clinical use in caries management, combining antimicrobial action with aesthetic preservation.
METHODS: SCF was synthesized by reacting 1,3,5-triaza-7-phosphaadamantane (PTA) with silver fluoride and characterised by electrospray ionization mass spectrometry. Silver and fluoride concentrations were measured by inductively coupled plasma atomic emission spectroscopy and fluoride ion-specific electrode. Alkalinity was measured using a pH meter. Cytotoxicity was assessed by determining the half maximal inhibitory concentration (IC50) against the human gingival fibroblasts (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Demineralised human dentine blocks were treated with SCF, incubated for 7 days with Streptococcus mutans, and assessed for antibacterial activity and discolouration. Biofilm kinetics and viability were analysed via colony-forming unit (CFU) counts and confocal laser scanning microscopy (CLSM). Dentine discolouration was quantified using colour spectrophotometry. Controls included 38% silver diamine fluoride (SDF; positive) and water (negative).
RESULTS: At 180 days, 55% SCF solution contained 127,498 ± 3,535 ppm silver and 27,215 ± 118 ppm fluoride, with a pH of 10. SCF demonstrated higher IC50 values compared to SDF in HGF-1 (35.5 ± 1.1 ppm vs. 17.7 ± 0.5 ppm; p < 0.001) and SHED (95.5 ± 0.1 ppm vs. 6.4 ± 0.3 ppm; p < 0.001). Log CFU counts for the SCF, SDF, and Water groups were 4.2 ± 0.2, 3.8 ± 0.4 and 5.2 ± 0.2 (SCF, SDF<Water, p < 0.001). CLSM showed dead-to-live ratios of SCF, SDF and Water group were 1.0 ± 0.3, 1.4 ± 0.5, and 0.2 ± 0.1 (p < 0.001; SCF, SDF > Water). Spectrophotometric analysis revealed ΔE values of SCF, SDF and Water groups were 5.8 ± 3.3, 73.0 ± 17.6, and 5.3 ± 2.7 (p < 0.001; SCF, Water < SDF).
CONCLUSION: A novel SCF solution with potent antibacterial efficacy and minimal discolouration was successfully developed.
CLINICAL SIGNIFICANCE: SCF demonstrates promising potential for clinical use in caries management, combining antimicrobial action with aesthetic preservation.
Предметные рубрики MeSH
HumansSilver CompoundsStreptococcus mutansCariostatic AgentsDentinDental CariesFluoridesStem CellsFibroblastsBiofilmsQuaternary Ammonium CompoundsMicroscopy, ConfocalGingivaTooth, DeciduousHydrogen-Ion ConcentrationAnti-Bacterial AgentsSilverSpectrophotometry, AtomicTooth DiscolorationCell SurvivalMicrobial ViabilityInhibitory Concentration 50Fluorides, TopicalMicrobial Sensitivity TestsColony Count, MicrobialCells, CulturedSpectrometry, Mass, Electrospray Ionization