Automatic disposable microfluidic cartridge system for ELISA on microbeads with electrochemical detection.

A closed microfluidic system was developed, featuring a multilayer cartridge for automated microparticle-based enzyme-linked immunoassay with electrochemical detection. This platform integrates analytical and engineering innovations to perform a complete assay workflow without manual intervention. Precision fluid control is achieved through two rotary valves that prevent solution cross-contamination while enabling reliable reagent delivery. Clinical validation using human serum samples containing N-terminal prohormone of brain natriuretic peptide demonstrated good linear correlation (R2 = 0.88) between electrochemical signal intensity and peptide concentration, as determined by reference method (Mindray BS-800), with a limit of detection of 0.720 ng/mL across the 0-30 ng/mL range. The short total analysis time (25 min) confirms the system's suitability for rapid clinical testing.