Effect of vaccination on expression of intracellular adhesion molecules 1 and vascular cell adhesion molecules 1 in murine schistosomiasis.

This work studied the approach of vaccination by utilizing ultraviolet attenuated S. mansoni cercariae on two surface adhesion molecules expression, intercellular adhesion molecules 1 (ICAM-1) and vascular cell adhesion molecules 1(VCAM-1). ICAM-1 and VCAM-1 expression were studied in livers of 37 CD-1 female mice (2 months aged and about 30 gm waited) which were divided into 3 groups. Analysis of adhesion molecules expression was done by direct immunofluorescence staining and quantitative measurement of positive signals by image analyzer. The main findings were: (1) ICAM-1 and VCAM-1 expression was mainly localized on granuloma cells. (2) The video count areas that expressed ICAM-1 and VCAM-1 were markedly increased in vaccinated than naive animals. The ICAM-1 intensity was (58.7%) in VC while (31.1%) in NC mice group. (3) Similarly the intensity of VCAM-1 expression was (73%) In VC while (54.2%) in NC. (4) The % change in ICAM-I expression relative to naive mice group was (326.9%) and (704.7) in NC and VC mice group respectively. (5) The % change in VCAM-1 expression in vaccinated animals relative to naive animals was (67,700) and (91,175) in NC and VC mice groups respectively.