Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics · 2008
Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide-induced interleukin-8 expression in human pulp cells.
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Min Kyung-San, Kim Hyun-Il, Chang Hoon-Sang, Kim Hyung-Ryong, Pae Hyun-Ock, Chung Hun-Taeg, Hong Seung-Heon, Shin Hong-In, Hong Chan-Ui, Lee Suk-Keun, Kim Eun-Cheol
Аннотация
OBJECTIVE: This study examined the effect of nitric oxide (NO) on interleukin-8 (IL-8) production and the involvement of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappaB) signaling pathways in primary cultured human pulp cells.
STUDY DESIGN: IL-8 production was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAPK activation and IkappaB degradation and phosphorylation were determined by western blotting.
RESULTS: Sodium nitroprusside (SNP), an NO donor, has increased IL-8 secretion and mRNA expression in a dose- and time-dependent manner. SNP induced the phosphorylation of p38 MAPK and extracellular-regulated kinase (ERK), degradation and phosphorylation of IkappaB, and activation of NF-kappaB. Furthermore, inhibition of the ERK, p38, and NF-kappaB pathways blocked SNP-induced IL-8 secretion.
CONCLUSION: Human pulp cells showed NO-induced IL-8 expression via the MAPK and NF-kappaB pathways, which may play an important role in the inflammatory responses of pulp and periapical lesions.
STUDY DESIGN: IL-8 production was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAPK activation and IkappaB degradation and phosphorylation were determined by western blotting.
RESULTS: Sodium nitroprusside (SNP), an NO donor, has increased IL-8 secretion and mRNA expression in a dose- and time-dependent manner. SNP induced the phosphorylation of p38 MAPK and extracellular-regulated kinase (ERK), degradation and phosphorylation of IkappaB, and activation of NF-kappaB. Furthermore, inhibition of the ERK, p38, and NF-kappaB pathways blocked SNP-induced IL-8 secretion.
CONCLUSION: Human pulp cells showed NO-induced IL-8 expression via the MAPK and NF-kappaB pathways, which may play an important role in the inflammatory responses of pulp and periapical lesions.
Предметные рубрики MeSH
Blotting, WesternCells, CulturedDental PulpEnzyme-Linked Immunosorbent AssayExtracellular Signal-Regulated MAP KinasesHumansI-kappa B ProteinsInterleukin-8MAP Kinase Signaling SystemNF-kappa BNitric OxideNitric Oxide DonorsNitroprussidePhosphorylationPulpitisReverse Transcriptase Polymerase Chain ReactionSignal Transductionp38 Mitogen-Activated Protein Kinases