Journal of ethnopharmacology · 2026
Prunella vulgaris L. ameliorates goiter in Wistar rats is associated with changes in Rap1GAP/CREB3L1/PI3K-AKT signaling pathway.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Han Guowei, He Xiaoqing, Zhang Chenyu, Lai Yaxin, Shan Zhongyan
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Prunella vulgaris L. (PV), known as Xia Ku Cao, has traditionally been used in Chinese medicine to dissipate nodules and reduce swelling and is widely used for thyroid disorders. However, its efficacy and mechanisms in iodine-deficiency-induced goiter remain unclear.
AIM OF THE STUDY: This study investigated the therapeutic effects of PV aqueous extract on iodine-deficiency-induced goiter and explored its potential molecular mechanisms.
MATERIALS AND METHODS: Forty-eight Wistar rats were used. Eight received a standard diet, whereas 40 received an iodine-deficient diet for 12 weeks and were subsequently assigned to the model, levothyroxine, or low-, medium-, and high-dose PV groups (n = 8). Treatments were administered orally for 16 weeks. Thyroid volume, thyroid weight, hormone levels, histopathology, ultrastructure, and hepatorenal biomarkers were evaluated. Chemical profiling and Deep DIA proteomics were performed, and selected proteins were validated by Western blotting.
RESULTS: PV extract contained rosmarinic acid, salvianic acid A, and other potentially bioactive constituents. Medium- and high-dose PV significantly reduced thyroid volume and attenuated thyroid inflammatory infiltration and ultrastructural injury. Proteomic and Western blot analyses showed that these effects were associated with increased Rap1GAP expression, decreased CREB3L1 expression, and reduced PI3K-AKT pathway activation. PV produced no detectable alterations in thyroid hormone levels or the measured hepatorenal biomarkers.
CONCLUSIONS: PV alleviated iodine-deficiency-induced goiter while preserving systemic thyroid function. Its effects were associated with modulation of the Rap1GAP/CREB3L1/PI3K-AKT signaling axis, supporting the traditional use of PV for thyroid enlargement.
AIM OF THE STUDY: This study investigated the therapeutic effects of PV aqueous extract on iodine-deficiency-induced goiter and explored its potential molecular mechanisms.
MATERIALS AND METHODS: Forty-eight Wistar rats were used. Eight received a standard diet, whereas 40 received an iodine-deficient diet for 12 weeks and were subsequently assigned to the model, levothyroxine, or low-, medium-, and high-dose PV groups (n = 8). Treatments were administered orally for 16 weeks. Thyroid volume, thyroid weight, hormone levels, histopathology, ultrastructure, and hepatorenal biomarkers were evaluated. Chemical profiling and Deep DIA proteomics were performed, and selected proteins were validated by Western blotting.
RESULTS: PV extract contained rosmarinic acid, salvianic acid A, and other potentially bioactive constituents. Medium- and high-dose PV significantly reduced thyroid volume and attenuated thyroid inflammatory infiltration and ultrastructural injury. Proteomic and Western blot analyses showed that these effects were associated with increased Rap1GAP expression, decreased CREB3L1 expression, and reduced PI3K-AKT pathway activation. PV produced no detectable alterations in thyroid hormone levels or the measured hepatorenal biomarkers.
CONCLUSIONS: PV alleviated iodine-deficiency-induced goiter while preserving systemic thyroid function. Its effects were associated with modulation of the Rap1GAP/CREB3L1/PI3K-AKT signaling axis, supporting the traditional use of PV for thyroid enlargement.