Journal of applied microbiology · 2025
Resistome and plasmidome genomic features of mcr-1.1-harboring Escherichia coli: a One Health approach.
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Simões de Oliveira Gabriela, Lentz Silvia Adriana Mayer, Müller Camila Zanfelice, Guerra Rafaela Ramalho, Dalmolin Tanise Vendruscolo, Volpato Fabiana Caroline Zempulski, de Lima-Morales Daiana, Lamb Wink Priscila, Barth Afonso Luís, Rabinowitz Peter, Martins Andreza Francisco
Аннотация
AIMS: This study evaluated the phenotypic and genotypic traits of mcr-1.1-harboring Escherichia coli isolates from chickens, pigs, humans, and farm environments. The resistome and the mobile genetic elements associated with the spread of mcr-1.1 in Southern Brazil were also characterized.
METHODS AND RESULTS: The 22 mcr-1.1-harboring E. coli isolates from different origins were selected for antimicrobial susceptibility testing and whole genome sequencing for characterization of the resistome, plasmids, and sequence types. All isolates presented several resistance genes and harbored the mcr-1.1 gene in a highly similar IncX4 plasmid. Furthermore, the mcr-1.1 gene co-occurred with the mcr-3.12 gene in a multidrug-resistant isolate from the farm environment.
CONCLUSIONS: These findings demonstrate that the mcr-1.1 gene in E. coli isolates from Brazil is spreading mainly by horizontal transfer of the IncX4 plasmid. The co-occurrence of mcr-1.1 and mcr-3.12 highlights pig farming as an important reservoir of colistin resistance.
METHODS AND RESULTS: The 22 mcr-1.1-harboring E. coli isolates from different origins were selected for antimicrobial susceptibility testing and whole genome sequencing for characterization of the resistome, plasmids, and sequence types. All isolates presented several resistance genes and harbored the mcr-1.1 gene in a highly similar IncX4 plasmid. Furthermore, the mcr-1.1 gene co-occurred with the mcr-3.12 gene in a multidrug-resistant isolate from the farm environment.
CONCLUSIONS: These findings demonstrate that the mcr-1.1 gene in E. coli isolates from Brazil is spreading mainly by horizontal transfer of the IncX4 plasmid. The co-occurrence of mcr-1.1 and mcr-3.12 highlights pig farming as an important reservoir of colistin resistance.
Предметные рубрики MeSH
Escherichia coliPlasmidsSwineAnimalsBrazilEscherichia coli ProteinsChickensHumansAnti-Bacterial AgentsEscherichia coli InfectionsColistinMicrobial Sensitivity TestsDrug Resistance, Multiple, BacterialOne HealthWhole Genome SequencingDrug Resistance, BacterialGene Transfer, HorizontalGenome, Bacterial