The prevalence, distribution, and diversity of Salmonella isolated from pork slaughtering processors and retail outlets in the Shandong Province of China.

Salmonella is a foodborne pathogen of global significance and is highly prevalent in pork. This study investigated the prevalence, contamination distribution, virulence genes and antibiotic resistance of Salmonella in 3 pork processors in the Shandong Province of China. Samples were collected from 13 different sampling sources across the slaughter procedures (600 samples) as well as at retail outlets supplied by these processors (45 samples). The prevalence was 18.9 % among all the samples, with the highest prevalence observed in feces (40.0 %), lairage pens (38.0 %), and hides (34.0 %). A total of 6 serotypes were identified, with S. Rissen (46.3 %) and S. typhimurium (32.0 %) found to be the most prevalent serotypes. 86.8 % of Salmonella isolates were multi-drug resistant, with the majority of strains resistant to erythromycin, sulfisoxazole, and ampicillin. The multilocus sequence typing analysis revealed 6 STs were obtained from 45 isolates, with the dominant type ST469 accounting for 40.0 % of the total, which suggested a high possibility of cross-contamination between the plant processing chain and retail outlets. This work reveals the prevalence and correlation of Salmonella isolates between pork slaughter and retail outlets and acts as a case-study for stakeholders wishing to improve pork supply chain hygiene, control cross-contamination between the various slaughtering processes, and obtain continuous updates on Salmonella surveillance.