Absence of hepatitis E virus in hedgehogs in northern Italy in 2022-2024.

Hepatitis E virus (HEV) causes viral hepatitis, posing a risk to vulnerable populations. Although swine are established reservoirs, the involvement of other animal species remains undefined. This study investigated 301 livers from naturally deceased European hedgehogs collected from wildlife centres (northern Italy), to determine their role in HEV transmission, spread and epidemiology, considering their proximity to urban areas. No positive samples were found by Real-time PCR, suggesting that hedgehogs play a marginal role in HEV epidemiology. Further studies are required to investigate other potential wild reservoirs to gain insight into HEV transmission dynamics, given the global impact of HEV-related diseases.