In vivo (Athens, Greece) · 2020
Cryopreservation of Biologically Functional Submandibular Gland Rudiments from Fetal Mice.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Adachi Keisuke, Ohno Yuta, Satoh Keitaro, Shitara Akiko, Muramathu Yasunori, Kashimata Masanori
Аннотация
BACKGROUND/AIM: Cryopreservation of cell lines has been widely used in the laboratory; however, cryopreservation of organs is still considered to be difficult. The submandibular gland (SMG) of fetal mice is one of the best-characterized organs. We investigated the conditions for cryopreserving SMG rudiments.
MATERIALS AND METHODS: Embryonic day 13 SMG rudiments were cryopreserved with or without a cryoprotectant. They were thawed and incubated in DMEM/F12 medium. Moreover, the influence of EGF stimulation on the signaling cascade after frozen-thawing the rudiments was analyzed by Western blotting.
RESULTS: When SMG rudiments were cryopreserved without a cryoprotectant, all cells in the rudiments died. However, the SMG rudiments that had been preserved in a cryoprotectant showed branching morphogenesis. Additionally, the responsiveness of signaling cascades to EGF did not differ between frozen with a cryoprotectant and non-frozen rudiments.
CONCLUSION: Cryopreservation might be a useful technology for preserving tissues from small organs, such as fetal SMG rudiments.
MATERIALS AND METHODS: Embryonic day 13 SMG rudiments were cryopreserved with or without a cryoprotectant. They were thawed and incubated in DMEM/F12 medium. Moreover, the influence of EGF stimulation on the signaling cascade after frozen-thawing the rudiments was analyzed by Western blotting.
RESULTS: When SMG rudiments were cryopreserved without a cryoprotectant, all cells in the rudiments died. However, the SMG rudiments that had been preserved in a cryoprotectant showed branching morphogenesis. Additionally, the responsiveness of signaling cascades to EGF did not differ between frozen with a cryoprotectant and non-frozen rudiments.
CONCLUSION: Cryopreservation might be a useful technology for preserving tissues from small organs, such as fetal SMG rudiments.