Comprehensive proteomic profile in newly diagnosed patients with inflammatory bowel disease: identification of potential biomarkers.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Baldán-Martín Montse, Iloro Ibon, Azkargorta Mikel, Soleto Irene, Orejudo Macarena, Ramirez Cristina, Mercado Jorge, Suárez-Trujillo Fabio, Sánchez-Sánchez Cristina, Garre Ana, Riestra Sabino, Rivero Montserrat, Gutiérrez Ana, Rodríguez-Lago Iago, Fernández-Salazar Luis, Ceballos Daniel, Benítez José Manuel, Aguas Mariam, Bastón-Rey Iria, Bermejo Fernando, Casanova María José, Lorente-Poyatos Rufo H, Ber Yolanda, Ginard Daniel, Esteve María, De Francisco Ruth, García María José, Francés Rubén, Cabriada Jose Luis, Soto Pilar, Nos Pilar, Acosta Manuel Barreiro-de, Guerra Iván, Cruz Daniel Hervías, Domínguez Cajal Manuel, Royo Vanesa, Aceituno Montserrat, Martín-Cófreces Noa B, Elortza Félix, Gisbert Javier P, Chaparro María
Аннотация
DESIGN: In the discovery phase, label-free quantitative proteomics was performed to analyze proteomic profiles in serum extracellular vesicles (EVs), serum, urine, and intestinal tissue from 100 newly diagnosed IBD patients (50 CD and 50 UC), and 51 healthy controls (HC). Serum candidate biomarkers were validated using ELISA in a separate subset cohort (87 CD, 134 UC, and 99 HC), and immunohistochemistry was performed on biopsies from the discovery cohort to confirm findings.
RESULTS: We identified 419 proteins in serum EVs, 468 in serum, 683 in urine, and 2603 in intestinal tissue. ELISA results showed lower levels of TTR and APOC3 and higher levels of ATRN in UC patients compared to HC. Similarly, CD patients showed lower TTR and higher ATRN levels compared to HC. Moreover, serum protein S10A9 was differentially upregulated in CD vs UC. Immunohistochemistry revealed increased PRDX4 and AZU1 expression in the ileum of CD patients, whereas AOFB expression was lower in the ileum of CD and in the left colon of both CD and UC compared to HC.
CONCLUSION: This comprehensive proteomic study has identified a set of proteins differentially expressed in IBD, which may contribute to a better understanding of its mechanisms and hold promise as candidate biomarkers. Although these findings are preliminary, they warrant further investigation to evaluate their diagnostic and therapeutic relevance.