BMC complementary and alternative medicine · 2015
Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line.
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Abdul Rahman Sazli Farahani, Jubri Zakiah, Abdul Rahman Mariati, Karsani Saiful Anuar, Md Top Abdul Gapor, Wan Ngah Wan Zurinah
Аннотация
BACKGROUND: To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells.
METHODS: HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS: GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment.
CONCLUSIONS: GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.
METHODS: HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS: GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment.
CONCLUSIONS: GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.
Предметные рубрики MeSH
Antineoplastic AgentsAntioxidantsBile Duct NeoplasmsChromansDown-RegulationElectrophoresis, Gel, Two-DimensionalHep G2 CellsHepatoblastomaHumansLiverLiver NeoplasmsPeroxiredoxinsProteinsProteomicsRNA, MessengerReal-Time Polymerase Chain ReactionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTocotrienolsUp-RegulationVitamin EVitamins