Orthodontics & craniofacial research · 2025
The NLRP3 Inflammasome Regulates Orthodontic Tooth Movement.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Latzko Lisa, Degenhart Gerald, Blumer Michael J, Gruber Reinhard, Haybäck Johannes, Manzl Claudia, Crismani Adriano G
Аннотация
OBJECTIVE: The NLRP3 inflammasome is a damage sensor driving pyroptotic cell death during orthodontic tooth movement (OTM). Recent studies have revealed impaired one-week OTM in mice lacking NLRP3. However, the relevance of NLRP3 during long-term OTM remains unknown.
MATERIALS AND METHODS: OTM was analysed after 7, 14 and 21 days of force application in mice lacking NLRP3 (NLRP3-/-), wild-type (WT) male and female littermates. Micro-computed tomography (μCT) was used to assess molar movement and bone quality. Tartrate-resistant acid phosphatase (TRAP) staining was used to visualise osteoclastic activity.
RESULTS: We show here that after 2 weeks of force application, OTM was delayed in mice lacking NLRP3 compared to WT mice. Interestingly, OTM regained speed in NLRP3-/- mice catching up with the WT controls at day 21. Independent of NLRP3 expression, OTM caused alveolar bone loss compared to the untreated contralateral side. NLRP3-/- mice showed a diminished number of TRAP-positive osteoclasts than WT male mice 14 and 21 days after OTM induction.
CONCLUSION: The results of this study suggest that NLRP3 is relevant during the early but not later stages of OTM.
MATERIALS AND METHODS: OTM was analysed after 7, 14 and 21 days of force application in mice lacking NLRP3 (NLRP3-/-), wild-type (WT) male and female littermates. Micro-computed tomography (μCT) was used to assess molar movement and bone quality. Tartrate-resistant acid phosphatase (TRAP) staining was used to visualise osteoclastic activity.
RESULTS: We show here that after 2 weeks of force application, OTM was delayed in mice lacking NLRP3 compared to WT mice. Interestingly, OTM regained speed in NLRP3-/- mice catching up with the WT controls at day 21. Independent of NLRP3 expression, OTM caused alveolar bone loss compared to the untreated contralateral side. NLRP3-/- mice showed a diminished number of TRAP-positive osteoclasts than WT male mice 14 and 21 days after OTM induction.
CONCLUSION: The results of this study suggest that NLRP3 is relevant during the early but not later stages of OTM.