Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2025
[Effect of dental-derived stem cells on the biological characteristics of cementoblasts].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Li Wenwen, Ma Wensheng, Li Yinghui, Zhao Yuan, Li Dongna, Zhai Haoyan, Liu Chunyan
Аннотация
PURPOSE: To investigate the effect of dental-derived mesenchymal stem cells on the biological characteristics of cementoblasts.
METHODS: Periodontal ligament stem cells (PDLSCs) and dental pulp stem cell (DPSCs) were isolated, flow cytometry was used to detect cell surface markers. Cementoblasts OCCM-30 were coculture with PDLSCs and DPSCs for 12 h and 24 h. Cell proliferation was examined using the CCK-8 method. ALP staining was used to evaluate the osteogenic differentiation of cementoblasts in different treatment groups. Wound healing migration assays and Transwell migration assays were performed to compare the migration of cementoblasts. The expression of MMP9 and MMP13 was assessed using enzyme-linked immunosorbent assay(ELISA).
RESULTS: The expression rates of CD90 and CD34 of PDLSCs were 99.44% and 0.23%. The expression rates of CD90 and CD34 of DPSCs were 99.93% and 3.47%. Their multipotency were illustrated based on differentiation to abiogenic, osteogenic lineages. Human mesenchymal stem cells promoted the proliferation of cementoblasts(P＜0.05). The osteogenic differentiation of cementoblasts was increased after stem cells treatment. The migration of cementoblasts was increased after stem cells treatment (P＜0.05), and the expression of MMP9 and MMP13 were enhanced.
CONCLUSIONS: Dental-derived stem cells can promote the proliferation, osteogenic differentiation and migration of cementoblasts, playing an active role in cementum restoration and providing a scientific basis for promoting the repair process in root resorption.
METHODS: Periodontal ligament stem cells (PDLSCs) and dental pulp stem cell (DPSCs) were isolated, flow cytometry was used to detect cell surface markers. Cementoblasts OCCM-30 were coculture with PDLSCs and DPSCs for 12 h and 24 h. Cell proliferation was examined using the CCK-8 method. ALP staining was used to evaluate the osteogenic differentiation of cementoblasts in different treatment groups. Wound healing migration assays and Transwell migration assays were performed to compare the migration of cementoblasts. The expression of MMP9 and MMP13 was assessed using enzyme-linked immunosorbent assay(ELISA).
RESULTS: The expression rates of CD90 and CD34 of PDLSCs were 99.44% and 0.23%. The expression rates of CD90 and CD34 of DPSCs were 99.93% and 3.47%. Their multipotency were illustrated based on differentiation to abiogenic, osteogenic lineages. Human mesenchymal stem cells promoted the proliferation of cementoblasts(P＜0.05). The osteogenic differentiation of cementoblasts was increased after stem cells treatment. The migration of cementoblasts was increased after stem cells treatment (P＜0.05), and the expression of MMP9 and MMP13 were enhanced.
CONCLUSIONS: Dental-derived stem cells can promote the proliferation, osteogenic differentiation and migration of cementoblasts, playing an active role in cementum restoration and providing a scientific basis for promoting the repair process in root resorption.