Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2016
[Sex bias in generation and functional phenotypes of peripheral T follicular helper cells in schistosomiasis japonica].
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Lei Xu, Xiang Qian, Jiao Jing, Ya-Lin Li, Yang Zhang, Wei Li, Xin Jin, Li-Yang Dong, Sha Zhou, Ji-Feng Zhu, Feng Liu, Xiao-Jun Chen, Chuan Su
Аннотация
OBJECTIVE: To study the influence of sex on the generation and function of T follicular helper (Tfh) cells in the process of Schistosoma japonicum infection.
METHODS: Totally 50 schistosomiasis patients were selected in the endemic areas of Chizhou City, Anhui Province, and totally 50 healthy people were selected in the non-endemic areas of Chizhou City as the controls. The peripheral bloods were collected from the above study subjects, and the human peripheral blood mononuclear cells from male/female schistosomiasis japonica patients or healthy controls were collected into the sodium heparin tubes and purified by Ficoll-paque plus density gradient centrifugation. Then, the percentages of total Tfh cell, ICOS+ Tfh cells, and PD-1+ Tfh cells in the schistosomiasis male/female patients or healthy controls were evaluated by flow cytometry.
RESULTS: Compared with the male and female healthy controls, the frequency of the total Tfh cells (Umale = 149.0, Ufemale = 39.5, both P < 0.01), the percentages of PD-1+ Tfh cells (tmale = 5.9, tfemale = 7.7, both P < 0.01) and ICOS+ Tfh cells (tmale = 3.2, tfemale = 5.9, both P < 0.01) of the male and female patients with schistosomiasis increased, all the differences were statistically significant. The further analysis showed that the percentage of total Tfh cells (U = 187.5, P < 0.05), ICOS+ Tfh cells (t = 3.2, P < 0.05), and PD-1+ Tfh cells (t = 3.0, P < 0.05) of the female patients were markedly higher than those of the male patients, all the differences were also statistically significant.
CONCLUSIONS: Sex may be a crucial role in the generation and function of Tfh cells in the process of Schistosoma japonicum infection.
METHODS: Totally 50 schistosomiasis patients were selected in the endemic areas of Chizhou City, Anhui Province, and totally 50 healthy people were selected in the non-endemic areas of Chizhou City as the controls. The peripheral bloods were collected from the above study subjects, and the human peripheral blood mononuclear cells from male/female schistosomiasis japonica patients or healthy controls were collected into the sodium heparin tubes and purified by Ficoll-paque plus density gradient centrifugation. Then, the percentages of total Tfh cell, ICOS+ Tfh cells, and PD-1+ Tfh cells in the schistosomiasis male/female patients or healthy controls were evaluated by flow cytometry.
RESULTS: Compared with the male and female healthy controls, the frequency of the total Tfh cells (Umale = 149.0, Ufemale = 39.5, both P < 0.01), the percentages of PD-1+ Tfh cells (tmale = 5.9, tfemale = 7.7, both P < 0.01) and ICOS+ Tfh cells (tmale = 3.2, tfemale = 5.9, both P < 0.01) of the male and female patients with schistosomiasis increased, all the differences were statistically significant. The further analysis showed that the percentage of total Tfh cells (U = 187.5, P < 0.05), ICOS+ Tfh cells (t = 3.2, P < 0.05), and PD-1+ Tfh cells (t = 3.0, P < 0.05) of the female patients were markedly higher than those of the male patients, all the differences were also statistically significant.
CONCLUSIONS: Sex may be a crucial role in the generation and function of Tfh cells in the process of Schistosoma japonicum infection.