Germs · 2019
Diversity of toxin genotypes and antimicrobial susceptibility of Clostridium perfringens isolates from feces of infants.
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Al Radaideh Alaa J, Badran Eman F, Shehabi Asem A
Аннотация
INTRODUCTION: This study investigated the most important epidemiological characteristics of C. perfringens strains colonizing the intestine of Jordanian infants.
METHODS: A total of 302 fecal samples were collected from Jordanian infants aged ≤ 1 year from patients hospitalized in the neonatal intensive care unit and from the outpatient department. Samples were cultured for detection of C. perfringens and evaluation of their antimicrobial resistance; identification of their potential toxins genes was performed using PCR.
RESULTS: Overall the C. perfringens colonization rate was 27.2% (82/302). Infants aged ≤ 6 months showed significantly higher (p<0.004) colonization than older infants. The occurrence rates of C. perfringens isolates carrying potential specific toxin genes were as follows: alpha toxin 95.1% (78/82), beta-2 toxin 69.5% (57/82), beta toxin 14.6% (12/82), and only 2.4% (2/82) epsilon toxin. No isolate carried the iota toxin. Genotype A was the most prevalent among C. perfringens isolates (78.1%). The antimicrobial resistance rates of C. perfringens isolates were 20% to metronidazole and erythromycin, 16.7% to levofloxacin, and 6.7% to vancomycin.
CONCLUSIONS: This study demonstrates that the majority of C. perfringens isolates from feces of Jordanian infants were classified as type A, few isolates were classified as type C and type D, and all were negative for potential enterotoxin genes causing diarrhea.
METHODS: A total of 302 fecal samples were collected from Jordanian infants aged ≤ 1 year from patients hospitalized in the neonatal intensive care unit and from the outpatient department. Samples were cultured for detection of C. perfringens and evaluation of their antimicrobial resistance; identification of their potential toxins genes was performed using PCR.
RESULTS: Overall the C. perfringens colonization rate was 27.2% (82/302). Infants aged ≤ 6 months showed significantly higher (p<0.004) colonization than older infants. The occurrence rates of C. perfringens isolates carrying potential specific toxin genes were as follows: alpha toxin 95.1% (78/82), beta-2 toxin 69.5% (57/82), beta toxin 14.6% (12/82), and only 2.4% (2/82) epsilon toxin. No isolate carried the iota toxin. Genotype A was the most prevalent among C. perfringens isolates (78.1%). The antimicrobial resistance rates of C. perfringens isolates were 20% to metronidazole and erythromycin, 16.7% to levofloxacin, and 6.7% to vancomycin.
CONCLUSIONS: This study demonstrates that the majority of C. perfringens isolates from feces of Jordanian infants were classified as type A, few isolates were classified as type C and type D, and all were negative for potential enterotoxin genes causing diarrhea.