Wei sheng yan jiu = Journal of hygiene research · 2016
[Preliminary study on preservation and preparation of Giardia standard body by ~(60)Co irradiation].
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Zhang Xiao, Sun Zongke, Ding Cheng, Li Xia
Аннотация
OBJECTIVE: Preliminary study on preparation methods of Giardia standard body by~(60)Co devitalized and the preservation conditions.
METHODS: Giardia irradiated by different doses of~(60)Co 0-1200 Gy and assessed by immunofluorescence assay. In the same time, the inactivated Giardia were preserved in 2. 5% K2Cr2O7 at 4℃. Then the Giardia by immunofluorescence assay was observed over the next 5 months.
RESULTS: After treatment with 900 Gy of~(60)Co radiation, 91. 44% ± 1. 64% of the cysts were inactivated. Moreover, indicators of cysts meet the requirements. After stored at 4 ℃ for 4 months, the number of total cysts, inactivation ratio and the morphology of cysts were meet the requirement.
CONCLUSION: It is an effective method to inactivate Giardia cysts with 900 Gy irradiation of~(60)Co. And Giardia cysts can be preserved in 2. 5% K2Cr2O7 at 4 ℃ for 4months.
METHODS: Giardia irradiated by different doses of~(60)Co 0-1200 Gy and assessed by immunofluorescence assay. In the same time, the inactivated Giardia were preserved in 2. 5% K2Cr2O7 at 4℃. Then the Giardia by immunofluorescence assay was observed over the next 5 months.
RESULTS: After treatment with 900 Gy of~(60)Co radiation, 91. 44% ± 1. 64% of the cysts were inactivated. Moreover, indicators of cysts meet the requirements. After stored at 4 ℃ for 4 months, the number of total cysts, inactivation ratio and the morphology of cysts were meet the requirement.
CONCLUSION: It is an effective method to inactivate Giardia cysts with 900 Gy irradiation of~(60)Co. And Giardia cysts can be preserved in 2. 5% K2Cr2O7 at 4 ℃ for 4months.