Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2010
[Autophagy is involved in 6-OHDA-induced dopaminergic cell death].
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Xia Li-ping, Li Ling-yun, Fei Xi-feng, Liang Zhong-qin
Аннотация
OBJECTIVE: To study the role of autophagy in the death of dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA).
METHODS: Rat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay.
RESULTS: Under TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group.
CONCLUSIONS: The increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.
METHODS: Rat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay.
RESULTS: Under TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group.
CONCLUSIONS: The increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.