Assessment of surface roughness and microbiological retention on custom abutments produced by different techniques and materials.

The aims of this study were twofold: first, to investigate the surface roughness of different abutment materials prepared using various manufacturing methods; and, second, to evaluate colonization by Streptococcus mutans and Candida albicans according to abutment material and manufacturing method. Six material/manufacturing method combinations were investigated in this study, namely chromium-cobalt (Cr-Co) (prepared using casting, milling, and laser sintering) and titanium, zirconia, and anodized titanium (all prepared using milling); titanium (stock) abutments were used as the control group. Surface roughness of seven specimens from each group was evaluated using atomic force microscopy and scanning electron microscopy. Laser-sintered Cr-Co had the lowest values of Ra (mean ± SD = 4.8 ± 0.8  nm), Rq (mean ± SD = 7.0 ± 1.2 nm), and Rmax (mean ± SD = 133.5 ± 31.7 nm), whereas milled zirconia had the highest values of Ra (mean ± SD = 112.9 ± 44.2 nm), Rq (mean ± SD = 142.8 ± 54.0 nm), and Rmax (mean ± SD = 1,035.7 ± 350.4 nm). Three specimens from each group were infected with S. mutans and three with C. albicans, and microbial counts were evaluated after culture. Colonization of Streptococcus mutans was highest on milled zirconia (mean log10 count ± SD = 5.87 ± 0.08) and lowest on milled Cr-Co (mean log10 count ± SD = 4.04 ± 0.11). For C. albicans, colonization was highest on milled titanium stock (mean log10 count ± SD = 6.62 ± 0.03) and lowest on milled anodized titanium (mean log10 count ± SD = 6.13 ± 0.03). Differences in surface roughness and microbial colonization among groups can aid clinicians in selecting materials based on clinical relevance, considering their potential impact on outcomes.