Enhanced dextran production by Weissella confusa in co-culture with Candida shehatae and its quorum sensing regulation mechanism.

Lactic acid bacteria (LAB) are well-known for its expertise in synthesizing exopolysaccharides (EPS), which are linked to significant health benefits, such as its prebiotic effects and ability to modulate the immune system. However, the synthesis of EPS is hindered by low yields. The objective of this study was to investigate the impact of co-cultivation on EPS output by Weissella confusa XG-3 when paired with Candida shehatae. The structure and characteristics of the purified EPS were analyzed. Gel permeation chromatography (GPC) revealed a molecular weight of 3.45 × 106 Da. High-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy and fourier transform infrared (FT-IR) spectroscopy showed that the EPS was a linear homopolysaccharide, mainly composed of glucose units, with a proposed structure of dextran featuring α-(1, 6) glycosidic linkages and occasional α-(1,3) branching. Fermentation processes were conducted in both mono-culture and co-culture settings, with tracking of pivotal gene expression. The result showed that the expression of luxS, ackA and wzb was significantly up-regulated in the co-culture system, which was positively correlated with dextransucrase activity and dextran production. C. shehatae promoted the growth of W. confusa XG-3 by consuming organic acids in the culture system through direct contact, initiating quorum sensing (QS), inducing dsr expression, increasing dextransucrase activity, and ultimately promoting dextran synthesis.