Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.] · 2026
Application of Baicalein Solution as a Novel Antioxidant After Bleaching: Analysis of Its Effects on Immediate Color Stability and the Reestablishment of Bond Strength in Bleached Enamel.
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da Silva Herica Myllena Correia, Pin Weslley Felipe, Santi Marina Rodrigues, Sahadi Beatriz Ometto, Martins Luís Roberto Marcondes
Аннотация
OBJECTIVE: To evaluate the antioxidant capacity of the baicalein solution (BA), as well as its effect when applied for different times to the surface of bleached dental enamel, considering immediate color stability and bond strength.
MATERIALS AND METHODS: Bovine enamel/dentin specimens (4 × 4 × 3 mm) were randomly divided into five groups (n = 10): Positive Control (PC): non-bleached, restored teeth; Negative Control (NC): bleached, restored teeth; and BA5, BA10, BA15 groups, with BA applied for 5, 10, or 15 min after bleaching with HP35%, followed by immediate restoration with Filtek Z350XT composite resin. The antioxidant activity (AA%) was evaluated using the DPPH test. Immediate color stability was assessed by ΔE00 and ΔWID at T1 (post-bleaching) and T2 (post-BA application), with repeated measurements at 24 h (T3) and 7 days (T4). Micro-shear bond strength (SBS) was evaluated at 24 h and 7 days. Statistical analysis was performed at a 5% significance level. SBS data were analyzed using two-way ANOVA, ΔE00 with generalized linear models, and ΔWID with Kruskal-Wallis followed by Dunn's post hoc test. Antioxidant activity and failure modes were analyzed descriptively.
RESULTS: The BA solution demonstrated an AA% of 97%. The final analyses (T4) of ΔE00 revealed that BA10 and BA15 presented statistically similar values to NC (p > 0.5), while BA5 exhibited the lowest ΔE00 among the groups (p < 0.05). For ΔWID (T4), all groups showed values similar to NC (p > 0.05). The application of BA improved SBS values at all time points (p < 0.05), with adhesive failures being predominant, followed by mixed failures.
CONCLUSION: Baicalein increased bond strength without compromising bleaching outcomes.
CLINICAL RELEVANCE: The use of BA showed promising results, indicating its potential as a novel agent to be further studied.
MATERIALS AND METHODS: Bovine enamel/dentin specimens (4 × 4 × 3 mm) were randomly divided into five groups (n = 10): Positive Control (PC): non-bleached, restored teeth; Negative Control (NC): bleached, restored teeth; and BA5, BA10, BA15 groups, with BA applied for 5, 10, or 15 min after bleaching with HP35%, followed by immediate restoration with Filtek Z350XT composite resin. The antioxidant activity (AA%) was evaluated using the DPPH test. Immediate color stability was assessed by ΔE00 and ΔWID at T1 (post-bleaching) and T2 (post-BA application), with repeated measurements at 24 h (T3) and 7 days (T4). Micro-shear bond strength (SBS) was evaluated at 24 h and 7 days. Statistical analysis was performed at a 5% significance level. SBS data were analyzed using two-way ANOVA, ΔE00 with generalized linear models, and ΔWID with Kruskal-Wallis followed by Dunn's post hoc test. Antioxidant activity and failure modes were analyzed descriptively.
RESULTS: The BA solution demonstrated an AA% of 97%. The final analyses (T4) of ΔE00 revealed that BA10 and BA15 presented statistically similar values to NC (p > 0.5), while BA5 exhibited the lowest ΔE00 among the groups (p < 0.05). For ΔWID (T4), all groups showed values similar to NC (p > 0.05). The application of BA improved SBS values at all time points (p < 0.05), with adhesive failures being predominant, followed by mixed failures.
CONCLUSION: Baicalein increased bond strength without compromising bleaching outcomes.
CLINICAL RELEVANCE: The use of BA showed promising results, indicating its potential as a novel agent to be further studied.