Zhonghua nan ke xue = National journal of andrology · 2004
[Biological characteristics of female rabbit clitoral smooth muscle cell culture in vitro].
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Liu Bo, Liu Jihong, Wang Tao
Аннотация
OBJECTIVE: To investigate the method of culturing rabbit clitoral smooth muscle cells(SMC) in vitro and their biological characteristics.
METHODS: Rabbit clitoral SMCs were cultured in vitro by enzymatically dispersed method, and morphological observation, cell counting and immunohistochemical staining were employed to study their biological characteristics.
RESULTS: Rabbit clitoral SMCs were spindle-shaped and parallelled along their longitudinal axis, and the attachment and proliferation in vitro were rapid.
CONCLUSION: Rabbit clitoral SMCs cultured in vitro can grow and maintain their steady characteristics when provided with appropriate culture condition. Clitoral SMCs cultured in vitro can serve as a cell material for researches of clitoris.
METHODS: Rabbit clitoral SMCs were cultured in vitro by enzymatically dispersed method, and morphological observation, cell counting and immunohistochemical staining were employed to study their biological characteristics.
RESULTS: Rabbit clitoral SMCs were spindle-shaped and parallelled along their longitudinal axis, and the attachment and proliferation in vitro were rapid.
CONCLUSION: Rabbit clitoral SMCs cultured in vitro can grow and maintain their steady characteristics when provided with appropriate culture condition. Clitoral SMCs cultured in vitro can serve as a cell material for researches of clitoris.