Медицинская библиотека · Tropical medicine & international health : TM & IH · 2010

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya.

Basiye Frank L, Mbuchi Margaret, Magiri Charles, Kirigi George, Deborggraeve Stijn, Schoone Gerard J, Saad Alfarazdeg A, El-Safi Sayda, Matovu Enock, Wasunna Monique K

Клиническое исследование II фазыСтатьяПоддержка исследования: вне правительства СШАПеревод пока не подготовлен

Аннотация

OBJECTIVE

To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya.

METHODS

Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays.

RESULTS

The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively.

CONCLUSION

Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease.

Для цитирования: Basiye Frank L, Mbuchi Margaret, Magiri Charles, Kirigi George, Deborggraeve Stijn, Schoone Gerard J, Saad Alfarazdeg A, El-Safi Sayda, Matovu Enock, Wasunna Monique K. Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya.. Tropical medicine & international health : TM & IH. 2010. DOI: 10.1111/j.1365-3156.2010.02548.x.

Предметные рубрики MeSH

Animals DNA, Protozoan Endemic Diseases Humans Kenya Leishmania donovani Leishmaniasis, Visceral Polymerase Chain Reaction RNA, Protozoan Reproducibility of Results Self-Sustained Sequence Replication Sensitivity and Specificity

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