[Preparation of anti-SEA egg yolk immunoglobulin and its preliminary application].

OBJECTIVE

To prepare the egg yolk immunoglobulin (IgY) against soluble egg antigen (SEA) of Schistosoma japonicum, and explore its feasibility for schistosomiasis screening in endemic areas.

METHODS

The urine samples of indirect haemagglutination test (IHA) positives from endemic areas and healthy people from non-endemic areas were collected. The Anti-SEA IgY was obtained through immunization by subcutaneous injection of SEA into Laihang hen, and its molecular weight was determined by SDS polyacrylamide gel electrophoresis (SDS-PAGE) . The circulating soluble egg antigen (CSEA) in the urine of IHA positives and healthy people were detected by ELISA-double antibody sandwich method with the Anti-SEA IgY as the capture antibody.

RESULTS

Anti-SEA IgY was prepared and purified successfully. Totally 48 urine samples of IHA positives were detected by ELISA and 26 of them were CSEA positives (54.17%); 10 urine samples of healthy people were detected, and all of them were negative.

CONCLUSIONS

The CSEA in human urine can be detected effectively by ELISA based on IgY. As a convenient and atraumatic method, it could be applied in schistosomiasis screening.