Enfermedades infecciosas y microbiologia clinica (English ed.) · 2019
Evaluation of the DNA microarray "AMR Direct Flow Chip Kit" for detection of antimicrobial resistance genes from Gram-positive and Gram-negative bacterial isolated colonies.
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Torres Fink Ignacio, Tormo Palop Nuria, Borrás Salvador Rafael, Buesa Gómez Javier, Gimeno Cardona Concepción, Navarro Ortega David
Аннотация
INTRODUCTION: The AMR Direct Flow Chip assay allows the simultaneous detection of a large variety of antibiotic resistance genetic markers. To assess this kit's performance, we use isolated colonies as starting material. The assay has been approved by the European Economic Area as a suitable device for in vitro diagnosis (CE IVD) using clinical specimens.
METHODS: A total of 210 bacterial isolates harbouring either one or more antimicrobial resistance genes including plasmid-encoded extended-spectrum β-lactamases (SHV, CTX-M) and carbapenemases (GES, SME, KPC, NMC/IMI, SIM, GIM, SPM, NDM, VIM, IMP, and OXA), mecA, vanA and vanB, and 30 controls were included.
RESULTS: The assay displayed a sensitivity and specificity of 100% for all target genes included in the array.
CONCLUSION: The AMR Direct Flow Chip Kit is an accurate assay for detecting genes which commonly confer resistance to β-lactams and vancomycin from isolated colonies in culture of Gram-positive and Gram-negative bacteria.
METHODS: A total of 210 bacterial isolates harbouring either one or more antimicrobial resistance genes including plasmid-encoded extended-spectrum β-lactamases (SHV, CTX-M) and carbapenemases (GES, SME, KPC, NMC/IMI, SIM, GIM, SPM, NDM, VIM, IMP, and OXA), mecA, vanA and vanB, and 30 controls were included.
RESULTS: The assay displayed a sensitivity and specificity of 100% for all target genes included in the array.
CONCLUSION: The AMR Direct Flow Chip Kit is an accurate assay for detecting genes which commonly confer resistance to β-lactams and vancomycin from isolated colonies in culture of Gram-positive and Gram-negative bacteria.
Предметные рубрики MeSH
Bacterial ProteinsDNA, BacterialDrug Resistance, MicrobialDrug Resistance, Multiple, BacterialGenes, BacterialGram-Negative BacteriaGram-Negative Bacterial InfectionsGram-Positive BacteriaGram-Positive Bacterial InfectionsHumansOligonucleotide Array Sequence AnalysisReagent Kits, DiagnosticSensitivity and SpecificityVancomycinbeta-Lactam Resistancebeta-Lactamases