[Establishment of rabbit with acute cerebral infarction by intervention and evaluation of computed tomographic perfusion imaging].

OBJECTIVE

To discuss the establishment of acute cerebral infarction model in rabbits and evaluate the value of computed tomographic perfusion (CTP) imaging.

METHODS

A total of 12 healthy adult New Zealand white rabbits were used. The model was established through femoral artery puncture and injecting autoblood clot into internal carotid artery through an inserted micro-catheter to occlude the artery. Digital subtraction angiography (DSA) cerebral angiography and CTP imaging were performed at 2 and 6 hours post-embolization.

RESULTS

Super-selective catheterization of internal carotid artery and an injection of auto-blood clots were successfully accomplished in all rabbits and the success rate was 100%. DSA showed that middle cerebral artery and anterior cerebral artery narrowed. At 2 hours post-embolism, CTP showed cerebral blood flow (CBF) and cerebral blood volume (CBV) decreased and mean transit time (MTT) and time to peak (TTP) increased in cerebral ischemia area. Compared with the contralateral mirror area, CBF and CBV decreased in infarct area and MTT and TTP elongated with significant statistical difference at 2 and 6 hours (P < 0.01).

CONCLUSION

The model of acute cerebral ischemia may be effectively established in rabbits. This micro-invasive technique is easily manipulated with a high success rate. And CTP is an effective way of diagnosing acute cerebral infarction.