Proceedings of the National Academy of Sciences of the United States of America · 2015

Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection.

СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен

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Mesquita Rafael D, Vionette-Amaral Raquel J, Lowenberger Carl, Rivera-Pomar Rolando, Monteiro Fernando A, Minx Patrick, Spieth John, Carvalho A Bernardo, Panzera Francisco, Lawson Daniel, Torres André Q, Ribeiro Jose M C, Sorgine Marcos H F, Waterhouse Robert M, Montague Michael J, Abad-Franch Fernando, Alves-Bezerra Michele, Amaral Laurence R, Araujo Helena M, Araujo Ricardo N, Aravind L, Atella Georgia C, Azambuja Patricia, Berni Mateus, Bittencourt-Cunha Paula R, Braz Gloria R C, Calderón-Fernández Gustavo, Carareto Claudia M A, Christensen Mikkel B, Costa Igor R, Costa Samara G, Dansa Marilvia, Daumas-Filho Carlos R O, De-Paula Iron F, Dias Felipe A, Dimopoulos George, Emrich Scott J, Esponda-Behrens Natalia, Fampa Patricia, Fernandez-Medina Rita D, da Fonseca Rodrigo N, Fontenele Marcio, Fronick Catrina, Fulton Lucinda A, Gandara Ana Caroline, Garcia Eloi S, Genta Fernando A, Giraldo-Calderón Gloria I, Gomes Bruno, Gondim Katia C, Granzotto Adriana, Guarneri Alessandra A, Guigó Roderic, Harry Myriam, Hughes Daniel S T, Jablonka Willy, Jacquin-Joly Emmanuelle, Juárez M Patricia, Koerich Leonardo B, Lange Angela B, Latorre-Estivalis José Manuel, Lavore Andrés, Lawrence Gena G, Lazoski Cristiano, Lazzari Claudio R, Lopes Raphael R, Lorenzo Marcelo G, Lugon Magda D, Majerowicz David, Marcet Paula L, Mariotti Marco, Masuda Hatisaburo, Megy Karine, Melo Ana C A, Missirlis Fanis, Mota Theo, Noriega Fernando G, Nouzova Marcela, Nunes Rodrigo D, Oliveira Raquel L L, Oliveira-Silveira Gilbert, Ons Sheila, Orchard Ian, Pagola Lucia, Paiva-Silva Gabriela O, Pascual Agustina, Pavan Marcio G, Pedrini Nicolás, Peixoto Alexandre A, Pereira Marcos H, Pike Andrew, Polycarpo Carla, Prosdocimi Francisco, Ribeiro-Rodrigues Rodrigo, Robertson Hugh M, Salerno Ana Paula, Salmon Didier, Santesmasses Didac, Schama Renata, Seabra-Junior Eloy S, Silva-Cardoso Livia, Silva-Neto Mario A C, Souza-Gomes Matheus, Sterkel Marcos, Taracena Mabel L, Tojo Marta, Tu Zhijian Jake, Tubio Jose M C, Ursic-Bedoya Raul, Venancio Thiago M, Walter-Nuno Ana Beatriz, Wilson Derek, Warren Wesley C, Wilson Richard K, Huebner Erwin, Dotson Ellen M, Oliveira Pedro L

Аннотация

Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (∼ 702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.

Предметные рубрики MeSH

Adaptation, Physiological Animals Base Sequence Chagas Disease Gene Transfer, Horizontal Host-Parasite Interactions Humans Insect Vectors Molecular Sequence Data Rhodnius Trypanosoma cruzi Wolbachia

Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection.

Ошибки и ограничения

Аннотация

Предметные рубрики MeSH