Journal of dentistry · 2025
Mineral-to-matrix ratio of caries-affected dentin treated with biomimetic analogs using the dentin fluid and a remineralizing solution as ion donors.
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Pires Maria Luiza Barucci Araujo, Mendes Soares Igor Paulino, Fernandes Lídia de Oliveira, Anselmi Caroline, Gibin Mariana Sversut, Baesso Mauro Luciano, Scheffel Débora Lopes Salles, de Souza Costa Carlos Alberto, Hebling Josimeri
Аннотация
OBJECTIVES: To evaluate the remineralizing potential of biomimetic analogs applied to caries-affected dentin (CAD) using the simulated body fluid (SBF) and a remineralization solution as Ca2+ and PO43- ions sources.
METHODS: Flat dentin surfaces from caries-free molars (N = 111) were subjected to a biological protocol (Streptococcus mutans) to induce dentin caries in vitro. The mineral and organic content of sound (SD), infected (ID), CAD, and CAD etched with phosphoric acid (CAD-PA) were determined to validate the protocol. After that, CAD-PA was treated with 100,000 µg/mL sodium trimetaphosphate (STMP) and 500 µg/mL polyacrylic acid (PAA), alone or combined (n = 6/group), applied for 1 min. Teeth were kept under intrapulpal pressure with SBF for 14 days. Next, the mineral-to-matrix ratio (MMR) and surface morphology were evaluated by ATR-FTIR and SEM. Statistical analyses were performed using RM-ANOVA/Sidak and Kruskal-Wallis (α = 5%).
RESULTS: The MMR was statistically different among the dentin substrates (p < 0.001). CAD showed a lower MMR than SD; the lowest MMR was seen when CAD was etched with PA (CAD-PA). However, no change in MMR was observed 14 days after the application of STMP, PAA, or STMP+PAA on CAD-PA.
CONCLUSIONS: The microbiological protocol produced a carious dentin that could be partially removed, resulting in a CAD-like substrate. Also, the application of the biomimetic analogs was ineffective in increasing the surface mineral content of the etched CAD after 14 days, even when a REM solution was used as a source of calcium and phosphate ions.
CLINICAL SIGNIFICANCE: The functional remineralization of caries-affected dentin using the dentin fluid and a mineralizing solution as sources of calcium and phosphate ions did not improve using biomimetic analogs of non-collagenous dentin proteins.
METHODS: Flat dentin surfaces from caries-free molars (N = 111) were subjected to a biological protocol (Streptococcus mutans) to induce dentin caries in vitro. The mineral and organic content of sound (SD), infected (ID), CAD, and CAD etched with phosphoric acid (CAD-PA) were determined to validate the protocol. After that, CAD-PA was treated with 100,000 µg/mL sodium trimetaphosphate (STMP) and 500 µg/mL polyacrylic acid (PAA), alone or combined (n = 6/group), applied for 1 min. Teeth were kept under intrapulpal pressure with SBF for 14 days. Next, the mineral-to-matrix ratio (MMR) and surface morphology were evaluated by ATR-FTIR and SEM. Statistical analyses were performed using RM-ANOVA/Sidak and Kruskal-Wallis (α = 5%).
RESULTS: The MMR was statistically different among the dentin substrates (p < 0.001). CAD showed a lower MMR than SD; the lowest MMR was seen when CAD was etched with PA (CAD-PA). However, no change in MMR was observed 14 days after the application of STMP, PAA, or STMP+PAA on CAD-PA.
CONCLUSIONS: The microbiological protocol produced a carious dentin that could be partially removed, resulting in a CAD-like substrate. Also, the application of the biomimetic analogs was ineffective in increasing the surface mineral content of the etched CAD after 14 days, even when a REM solution was used as a source of calcium and phosphate ions.
CLINICAL SIGNIFICANCE: The functional remineralization of caries-affected dentin using the dentin fluid and a mineralizing solution as sources of calcium and phosphate ions did not improve using biomimetic analogs of non-collagenous dentin proteins.