[The lipid metabolism based on the energy metabolism technology in obese mice induced by a high-fat diet].

OBJECTIVE

To ingvestigate effects of long-term high-fat diet on the energy and lipid metabolism.

METHODS

20 SPF male C57BL/6 mice were randomly divided into a control group fed a basic diet and a high-fat group fed a high-fat diet. Indirect calorimetry measurement was done to mearue total energy expenditure and metabolism in the mice at the 12th week. Multi-row quantitative CT was applied to detect body fat content and distribution in the mice. At the end of the experiment the mice were killed, and blood and liver were collected to detect the bio6hemical and histopathological indicators.

RESULTS

In comparison with the control group, the mice fed high-fat diet increased energy intake, body and visceral fat content and levels of fatty acid oxidation were significantlyincreased (P <0. 05). Fasting blood glucose, blood lipid, insulin and fatty acid content in liver and serum were significantly higher than those in the control group(P <0. 05). Pathology examinations showed hepatocytes steatosis, mitochondria and other organelles structural damage in the high-fat mice.

CONCLUSION

Long-term high-fat diet can lead to excessive energy intake, lipid dysmetabolism, abdominal obesity, hyperlipidemia, fatty liver and insulin resistance.