International archives of allergy and immunology · 2009
Detection of antigens reactive to IgE and IgA during wheat seed maturation and in different wheat cultivars.
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Constantin Claudia, Touraev Alisher, Heberle-Bors Erwin, Quirce Santiago, Huber Wolf Dietrich, Valenta Rudolf
Аннотация
BACKGROUND: Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease.
AIM: To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars.
METHODS: Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease.
RESULTS: With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable.
CONCLUSIONS: The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.
AIM: To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars.
METHODS: Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease.
RESULTS: With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable.
CONCLUSIONS: The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.