Oral diseases · 2025
Effects of Experimental Periodontitis and Periodontal Treatment on Alzheimer-Like Pathology in Rats.
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Yavuz Sedanur, Elibol Birsen, Demir Esra, Kinsiz Beyza, Toluk Ozlem, Gunpinar Sadiye
Аннотация
OBJECTIVE: This study investigated the effects of periodontitis (P) and non-surgical periodontal therapy (NSPT) on behavior, neurodegeneration, and neuroinflammation in rats with Alzheimer's disease (AD)-like pathology.
METHODS: AD-like pathology was induced in rats (n = 28) using STZ neurodegeneration model. Periodontitis was experimentally induced (n = 32), and half of which received NSPT with Chlorhexidine (CHX) gel. Behavioral assessment included the passive avoidance task (PA) and Morris water maze (MWM). Levels of NLRP3, phosphorylated tau (p-tau), and tau in the hippocampus, cerebrospinal fluid (CSF), and serum were measured by ELISA, while BACE1, IL1β, iNOS, and NF-κβ proteins were assessed by Western blotting.
RESULTS: Rats in the AD and AD + P groups performed worse in behavioral tests compared to controls (p < 0.05), whereas the NSPT group showed similar performance to controls (p > 0.05). CSF p-tau levels were comparable between AD and AD + P groups, but the hippocampal p-tau/tau ratio was significantly higher in the AD + P group (p < 0.05). BACE1 levels were similar in P and AD groups. NLRP3 and iNOS levels did not show significant differences across groups. Notably, the NSPT group exhibited reduced NF-κβ levels (p < 0.05).
CONCLUSIONS: Periodontitis may exacerbate AD-like molecular pathology, particularly by promoting tau hyperphosphorylation, while NSPT appears to mitigate disease progression and improve behavioral outcomes.
METHODS: AD-like pathology was induced in rats (n = 28) using STZ neurodegeneration model. Periodontitis was experimentally induced (n = 32), and half of which received NSPT with Chlorhexidine (CHX) gel. Behavioral assessment included the passive avoidance task (PA) and Morris water maze (MWM). Levels of NLRP3, phosphorylated tau (p-tau), and tau in the hippocampus, cerebrospinal fluid (CSF), and serum were measured by ELISA, while BACE1, IL1β, iNOS, and NF-κβ proteins were assessed by Western blotting.
RESULTS: Rats in the AD and AD + P groups performed worse in behavioral tests compared to controls (p < 0.05), whereas the NSPT group showed similar performance to controls (p > 0.05). CSF p-tau levels were comparable between AD and AD + P groups, but the hippocampal p-tau/tau ratio was significantly higher in the AD + P group (p < 0.05). BACE1 levels were similar in P and AD groups. NLRP3 and iNOS levels did not show significant differences across groups. Notably, the NSPT group exhibited reduced NF-κβ levels (p < 0.05).
CONCLUSIONS: Periodontitis may exacerbate AD-like molecular pathology, particularly by promoting tau hyperphosphorylation, while NSPT appears to mitigate disease progression and improve behavioral outcomes.
Предметные рубрики MeSH
AnimalsAlzheimer DiseasePeriodontitistau ProteinsMaleRatsHippocampusNitric Oxide Synthase Type IIDisease Models, AnimalAmyloid Precursor Protein SecretasesNLR Family, Pyrin Domain-Containing 3 ProteinChlorhexidineAspartic Acid EndopeptidasesNF-kappa BInterleukin-1betaRats, Sprague-DawleyPhosphorylationAvoidance Learning