[Correlation of CD269 Expression Patterns with Antigen Expression and Molecular Cytogenetics Abnormalities in Patients with Multiple Myeloma].

OBJECTIVE

To explore the correlation between CD269 expression patterns in multiple myeloma (MM) cells and the expression of other antigens and molecular cytogenetics.

METHODS

Flow cytometry was used to detect the expression level of CD269 in bone marrow plasma cells of 115 newly diagnosed MM patients. The patients were subsequently divided into CD269- group (<20%), CD269 partial expression group (20%-80%), and CD269 high expression group (>80%). The CD269 partial expression group and high expression group were combined as the CD269+ group. The immunophenotype of bone marrow plasma cells was analyzed by flow cytometry. Molecular cytogenetic analysis was performed using combined probe fluorescence in situ hybridization technology.

RESULTS

There were 27 patients (23.5%) in the CD269- group, 42 patients (36.5%) in the CD269 partial expression group, and 46 patients (40.0%) in the CD269 high expression group. The expression rate of CD56 antigen was 72.7% in the CD269+ group, which was higher than 48.1% in the CD269- group (P <0.05). The expression rates of CD56 and CD117 antigen in the CD269 high expression group were 76.1% and 47.8%, respectively, which were higher than 69.0% and 16.7% in the CD269 partial expression group (both P <0.05). The CD56/CD117 co-expression rate in the CD269 high expression group was 39.1%, which was higher than 14.8% in the CD269- group and 14.3% in the CD269 partial expression group (both P <0.05). The positive rate of IgH/CCND1 gene fusion in the CD269- group was 63.0%, which was higher than 31.0% in the CD269 partial expression group, 19.6% in the CD269 high expression group, and 25.0% in the CD269+ group (all P <0.05). The IgH deletion rate was 13.0% in the CD269 high expression group, while no IgH deletion was observed in the CD269 partial expression group, with a statistically significant difference between the two groups (P <0.05).

CONCLUSION

The expression of CD269 on bone marrow plasma cells in MM patients is significantly correlated with the expression of CD56 and CD117, as well as IgH/CCND1 gene fusion and IgH deletion. The high expression of CD269 is associated with better prognosis related biological indicators.