Detection of Group B Streptococcus in Vaginal Swab of Pregnant Women by Culture and PCR and Their Antibiotic Susceptibility Pattern in a Tertiary Care Hospital of Bangladesh.

Group B Streptococcus (GBS) is an important cause of neonatal infection and transmitted from colonized vagina of mother. The purpose of the present study was to see the status of GBS infection in pregnant women. This prospective cross sectional study was conducted from July 2013 to June 2014 at Dhaka Medical College Hospital, Dhaka, Bangladesh. Total 224 vaginal swab samples were studied. Organisms were isolated and identified by Wet film microscopy, Gram stain, biochemical tests, culture and PCR. Of the 224 samples, 46(20.53%) were GBS positive. Highest proportion (33.78%) of GBS was found in 20-29 years of age group. Regarding GBS positivity majority (43.47%) were second gravid, 82.60% in 35-37 weeks of pregnancy, 64.04% were from middle income group, 39.13% were oral contraceptive pill (OCP) users. Hundred percent GBS were sensitive to penicillin, ampicillin, ceftriaxone and vancomycin. Most (46.43%) of the GBS were resistant to gentamycin followed by 35.72% to doxycycline and 28.57% to chloramphenicol. The sensitivity of PCR was 100%. Prevalence of GBS colonization was 20.53% among the pregnant women of Dhaka Medical College Hospital signifies GBS infection might be a silent clinical problem.