Transactions of the Royal Society of Tropical Medicine and Hygiene · 2015
Food handlers as a link in the chain of transmission of Giardia duodenalis and other protozoa in public schools in southern Brazil.
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Colli Cristiane Maria, Bezagio Renata Coltro, Nishi Letícia, Ferreira Érika Cristina, Falavigna-Guilherme Ana Lúcia, Gomes Mônica Lúcia
Аннотация
BACKGROUND: Food handlers (FHs) may facilitate transmission and dissemination of pathogens. The importance of FHs as a link in the epidemiological chain of transmission of Giardia duodenalis and other intestinal protozoa was assessed.
METHODS: Fecal and subungual material from 27 FHs were analyzed using parasitological methods. G. duodenalis was identified by direct immunofluorescence and genotyped by PCR-RFLP for the bg and gdh genes, and gdh was sequenced.
RESULTS: At least one protozoan was detected in 30% (8/27) of the FHs and G. duodenalis (19%; 5/27) was the most common species. The AII and BIV genotypes were found in 20% (1/5) and 60% (3/5) of FHs infected with G. duodenalis, respectively.
CONCLUSIONS: FHs can be involved in the chain of transmission of G. duodenalis and other protozoa.
GENBANK ACCESSION NUMBERS: KJ741310 - KJ741313.
METHODS: Fecal and subungual material from 27 FHs were analyzed using parasitological methods. G. duodenalis was identified by direct immunofluorescence and genotyped by PCR-RFLP for the bg and gdh genes, and gdh was sequenced.
RESULTS: At least one protozoan was detected in 30% (8/27) of the FHs and G. duodenalis (19%; 5/27) was the most common species. The AII and BIV genotypes were found in 20% (1/5) and 60% (3/5) of FHs infected with G. duodenalis, respectively.
CONCLUSIONS: FHs can be involved in the chain of transmission of G. duodenalis and other protozoa.
GENBANK ACCESSION NUMBERS: KJ741310 - KJ741313.