The effects of pH on Ca2+-activated force in frog skeletal muscle fibers.

Ca2+-activated isometric force was recorded is skinned (sarcolemma mechanically removed) segments of frog skeletal muscle fibers immersed in bathing solution of different pH (5.0--10.5) and Ca2+ concentrations. Force in maximally activated fibers was near zero at pH 5.5, increased as pH increased to 7.5, remained relatively constant until pH 9.0 and then rapidly declined to zero by pH 10.5. The Ca2+ concentration at which 50% of maximum force was developed decreased 25-fold as pH increased from 5.5--7.5. The data also indicate that, while the fibers remains viable with acidosis, they deteriorate rapidly with alkalosis. These observations may be relevant clinically, since they parallel known effects of acidosis on cardiac contractility. The possible sites of action of H+ on the Ca2+-activated force generating mechanisms are discussed.