Detection of Microbial Contaminants in Water: Conventional Methods, Pragmatic Alternatives, and Nanosensing Techniques.

The complexities of microbial detection and conventional enumeration necessitates the adoption of pragmatic alternatives. This review expands the boundaries of knowledge for microbial detection and sensing, particularly within the field of water quality analysis. Observed alternatives to conventional techniques for microbial analyses in recent studies include Microarray, Fluorescent in situ hybridization (FISH), loop-mediated isothermal amplification (LAMP), matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and flow cytometry, while nanosensors stood out as an alternative for microbial detection in real-time. This study presents the limitation of conventional methods of detection in water and presents nanoparticles as a detection agent with possibility of incorporation into point-of-use detection. It is notable that nanosensors are currently emerging in the detection of bacteria, viruses and other pathogens in water and have been used in the detection of bacterial pathogens than viral. Nanosensors are established as good choice for rapid water analysis with application in point-of-use and analytical devices. In the use of nanozymes, the choice over natural enzymes can be linked to their unique and excellent catalytic activities, cost-effectiveness and ease of mass production.