[Effects of microRNA-218 on proliferation, apoptosis and invasion of human tongue cancer cell line SCC-4 and SCC-9].

PURPOSE: To study the effects of miR-218 on cell proliferation, apoptosis and invasion of human tongue cancer cell line SCC-4 and SCC-9 cells.

METHODS

SCC-4 and SCC-9 cells were transfected with negative control, miR-218 mimics and inhibitor, then cell proliferation was detected by MTT assay, cell cycle and cell apoptosis were measured by flow cytometry, cell invasion ability was tested by Transwell assay. The data were analyzed using GraphPad 7.0 software package.

RESULTS

Compared with control cells, there was no significant difference for cell proliferation, cell cycle, cell apoptosis and invasion ability in cells transfected with miR-218 inhibitor. However, decreased cell proliferation and invasion ability and increased apoptosis ratio were found in cells transfected with miR-218 mimics.

CONCLUSIONS

The expression level of miR-218 in tongue cancer cell lines may be correlated with cell proliferation, apoptosis and invasion.