Zhonghua zhong liu za zhi [Chinese journal of oncology] · 1997
[Mutation analysis of transforming growth factor-beta type II receptor gene in human gastric cancer tissues].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ke Y, Hagiwara K, Su X
Аннотация
OBJECTIVE: Mutations which cause abarrent expression of transforming type II receptor (TGF-beta RII) are responsible for the escape of some tumor cells from growth control midiated by TGF-beta. Two mutation hot spots (cDNA 709-718, 1931-1936) have been reported on human colon cancer cell lines. To elucidate the situation of this gene in human gastric cancer, 44 gastric cancer tissues were examined for mutation at the two hot spots.
METHODS: Genomic DNA extracted from the cancer tissues was PCR amplified and analysed by silver stain for single stranded conformation polymorphism. Positive samples were sequenced by dry-primer automatic sequencing method.
RESULTS: The data revealed a deletion rate of 6.8% (3/44) of the 1st hot spot; CA insertion of the 2nd hot spot was not found in all 44 gastric cancer tissues.
CONCLUSION: The mutation incidence of TGF-beta R II in human gastric cancer is low.
METHODS: Genomic DNA extracted from the cancer tissues was PCR amplified and analysed by silver stain for single stranded conformation polymorphism. Positive samples were sequenced by dry-primer automatic sequencing method.
RESULTS: The data revealed a deletion rate of 6.8% (3/44) of the 1st hot spot; CA insertion of the 2nd hot spot was not found in all 44 gastric cancer tissues.
CONCLUSION: The mutation incidence of TGF-beta R II in human gastric cancer is low.