Медицинская библиотека · Zhonghua zhong liu za zhi [Chinese journal of oncology] · 1997

[Mutation analysis of transforming growth factor-beta type II receptor gene in human gastric cancer tissues].

Ke Y, Hagiwara K, Su X

Аннотация на английскомСтатьяПеревод пока не подготовлен

Аннотация

OBJECTIVE

Mutations which cause abarrent expression of transforming type II receptor (TGF-beta RII) are responsible for the escape of some tumor cells from growth control midiated by TGF-beta. Two mutation hot spots (cDNA 709-718, 1931-1936) have been reported on human colon cancer cell lines. To elucidate the situation of this gene in human gastric cancer, 44 gastric cancer tissues were examined for mutation at the two hot spots.

METHODS

Genomic DNA extracted from the cancer tissues was PCR amplified and analysed by silver stain for single stranded conformation polymorphism. Positive samples were sequenced by dry-primer automatic sequencing method.

RESULTS

The data revealed a deletion rate of 6.8% (3/44) of the 1st hot spot; CA insertion of the 2nd hot spot was not found in all 44 gastric cancer tissues.

CONCLUSION

The mutation incidence of TGF-beta R II in human gastric cancer is low.

Для цитирования: Ke Y, Hagiwara K, Su X. [Mutation analysis of transforming growth factor-beta type II receptor gene in human gastric cancer tissues].. Zhonghua zhong liu za zhi [Chinese journal of oncology]. 1997.

Предметные рубрики MeSH

Humans Point Mutation Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Receptors, Transforming Growth Factor beta Stomach Neoplasms

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