'Multiple and short-range' cross-linking of dialdehyde carboxymethyl cellulose contributes to regulating the physicochemical property of collagen fibril.

Collagen fibril hydrogel (CH), with controllable micro-structure, sufficient modifying sites and excellent biocompatibility, has received widely attention in the regulation of biomacromolecules. Herein, dialdehyde carboxymethyl cellulose (DCMC) in different -CHO contents and molecular weights demonstrated two types of cross-linking behaviors to CH, 'limited and long-range' or 'multiple and short range' cross-linking, corresponding to -CHO content ranged from 0 to 53 % and 53 to 90 %, respectively. In regard of structure, non-destroying effect of DCMC on collagen was supported by FT-IR and XRD analysis. CH cross-linked by DCMC (CH-DC) showed declining porosity and aggregating fibrils as -CHO content of DCMC rising. In regard of physicochemical properties, DCMC with >53 % -CHO strengthened the hydrophilicity, thermal stability and degradation resistance of CH-DC. Also, there was 110 % growth on gel strength, 86 Pa enhancements on storage modulus, and 4.6 times decrease on the swelling ratio of CH-DC. Results indicated that DCMC with 79 % -CHO remarkably improved the physicochemical properties of CH via developing sufficient Schiff-base bonds with collagen fibril in a short distance. This study distinguished two patterns of DCMC cross-linking from physicochemical view. In other words, DCMC is potential to meet the requirement of protein-based materials with different expectations by adjusting its -CHO content and molecular weight.