Diagnosis of histoplasmosis by next-generation sequencing of microbial cell-free DNA in Texas.

We examine the impact of microbial cell-free DNA (mcfDNA) NGS for the diagnosis of Histoplasma capsulatuim in a quaternary referral center in Texas. Of eight included patients, five had a change in their antifungal coverage after mcfDNA NGS results with faster turnaround times than antigen testing and cultures.