Epidemiologia e servicos de saude : revista do Sistema Unico de Saude do Brasil · 2022
Self-collected nasopharyngeal swab and molecular test using pool testing as strategies to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): feasibility in medical students at the Universidade Federal de Minas Gerais, Brazil, 2021.
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Guimarães Nathalia Sernizon, Costa Murilo Soares, Machado Elaine Leandro, Sato Hugo Itaru, Amaral Eduarda de Carvalho Maia E, Arivabene Rafaela Galvão, Lourenço Karine Lima, Tupinambás Unaí, Fonseca Flávio Guimarães da, Takahashi Ricardo Hiroshi Caldeira, Teixeira Santuza Maria Ribeiro, Alves Claudia Regina Lindgren
Аннотация
OBJECTIVE: To show the feasibility of the combined use of self-collected nasopharyngeal swab and pool testing to detect SARS-CoV-2 in epidemiological surveys.
METHODS: This experience included a sample of 154 students at the Universidade Federal de Minas Gerais, who performed self-collected nasopharyngeal swab in individual cabins and without supervision. The molecular test was performed using the pool testing technique.
RESULTS: It took each person 5 minutes to collect the sample. An analysis was performed to detect endogenous RNA in 40 samples. The results showed that there were no failures resulting from self-collection. None of the pools detected the presence of viral RNA. The cost of molecular testing (RT-PCR), by pool testing, with samples obtained by self-collection was about ten times lower than the usual methods.
CONCLUSION: The strategies that were investigated proved to be economically feasible and valid for the research on SARS-CoV-2 in epidemiological surveys.
METHODS: This experience included a sample of 154 students at the Universidade Federal de Minas Gerais, who performed self-collected nasopharyngeal swab in individual cabins and without supervision. The molecular test was performed using the pool testing technique.
RESULTS: It took each person 5 minutes to collect the sample. An analysis was performed to detect endogenous RNA in 40 samples. The results showed that there were no failures resulting from self-collection. None of the pools detected the presence of viral RNA. The cost of molecular testing (RT-PCR), by pool testing, with samples obtained by self-collection was about ten times lower than the usual methods.
CONCLUSION: The strategies that were investigated proved to be economically feasible and valid for the research on SARS-CoV-2 in epidemiological surveys.