NF2 can mediate the expression of CAMK2A in a tissue specific manner.

Meningioma is the most prevalent primary intracranial tumor, with approximately half of patients harboring NF2 alteration. The rationale behind the presence of NF2 alteration in meningiomas and its absence in non-nerve system tumors remains elusive. Therefore, meningiomas and several non-nerve system tumor types were analyzed using KEGG analysis and CRISPR/Cas 9 technology to determine the role of NF2 in regulating tissue specificity. Moreover, the different regulatory patterns of Ca2+ and calcium/calmodulin-dependent protein kinase II alpha (CAMK2A) that play a decisive role in NF2 tissue-specific regulation were deciphered. Our results revealed that NF2 has a positive correlation in CAMK2A expression in both meningiomas and normal nervous system tissues but not in non-nervous system tumors and tissues, implying NF2 tissue-specificity is mediated by CAMK2A-related pathways. Thus, targeting CAMK2A may represent a promising strategy for drug screening and the development of therapeutics for NF2-associated meningiomas and other nervous system tumors.