Fabrication of NIR and ROS-responsive β-cyclodextrin/hyaluronic acid-based hybrid hydrogel for the treatment of HER2-positive gastric cancer cells.

The combined therapeutic approaches by unique biomaterial have great attention for gastric cancer therapy with peritoneal metastasis. In this research report, we designed NIR-responsive Fe3O4@MIL-100(Fe)-Upconversion nanoparticles (FMUNPs) functionalized injectable β-cyclodextrin/hyaluronic acid macromolecular hydrogel-encapsulated with HER-2 targeting trastuzumab (TZB) molecules to induce ROS against gastric cancer cells. The developed FMUNPs could provide effective ROS generation ability by photo-Fenton rection with NIR irradiation for toxicity and cell death against gastric cancer cells (NCI-N87). The ROS generation-induced apoptotic efficiency of the treated groups was confirmed by expressions of pro-apoptotic and anti-apoptotic markers. The TZB/FMUNPs-hydrogel with NIR prominently produced cell death by apoptotic pathway, which was confirmed by flow cytometry method. In-vivo study demonstrated that the significant reduction on tumor volume and size when tumor-induced mice treated with developed formulation with NIR irradiation. This investigation outcome demonstrates this novel formulation could be a new avenue for the gastric cancer treatment.