The Journal of antimicrobial chemotherapy · 2017
Isolation of azole-resistant Aspergillus fumigatus from the environment in the south-eastern USA.
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Hurst Steven F, Berkow Elizabeth L, Stevenson Katherine L, Litvintseva Anastasia P, Lockhart Shawn R
Аннотация
BACKGROUND: Azole resistance in isolates of the fungus Aspergillus fumigatus has been associated with agricultural use of azole fungicides. Environmental isolation of resistant isolates has been reported in Asia, Africa, Europe and South America.
OBJECTIVES: To determine whether A. fumigatus isolates containing TR34/L98H or TR46/Y121F/T289A can be found in fields in the USA treated with agricultural azoles.
METHODS: Crop debris was collected and screened for A. fumigatus. All A. fumigatus isolates were screened for azole resistance. The CYP51A gene of azole-resistant isolates was sequenced. The population structure of a subset of isolates was determined using microsatellite typing.
RESULTS: This article identifies azole-resistant A. fumigatus isolates containing the TR34/L98H mutation in an experimental peanut field that had been treated with azole fungicides.
CONCLUSIONS: These findings suggest the development of resistance to azole antifungals in A. fumigatus may be present where agricultural azoles are used in the USA.
OBJECTIVES: To determine whether A. fumigatus isolates containing TR34/L98H or TR46/Y121F/T289A can be found in fields in the USA treated with agricultural azoles.
METHODS: Crop debris was collected and screened for A. fumigatus. All A. fumigatus isolates were screened for azole resistance. The CYP51A gene of azole-resistant isolates was sequenced. The population structure of a subset of isolates was determined using microsatellite typing.
RESULTS: This article identifies azole-resistant A. fumigatus isolates containing the TR34/L98H mutation in an experimental peanut field that had been treated with azole fungicides.
CONCLUSIONS: These findings suggest the development of resistance to azole antifungals in A. fumigatus may be present where agricultural azoles are used in the USA.
Предметные рубрики MeSH
Antifungal AgentsAspergillosisAspergillus fumigatusAzolesCompostingCrops, AgriculturalCytochrome P-450 Enzyme SystemDrug Resistance, FungalFungal ProteinsFungicides, IndustrialGenotypeHigh-Throughput Nucleotide SequencingHumansMicrobial Sensitivity TestsMicrosatellite RepeatsMutationSoil MicrobiologyUnited States