Immunocytochemical localization of peptides in the endocrine pancreas of the snakes Vipera aspis and Natrix maura.

The endocrine pancreas of Vipera aspis and Natrix maura has been investigated by immunocytochemistry for the presence of six peptides reported to occur in Mammals or in Reptiles. Gastrin/CCK and PP were absent in the endocrine cells, VIP was located within nerve terminals only. Insulin, Glucagon, and Somatostatin were localized both in Vipera and Natrix, generally with a ring of D (Somatostatin) cells surrounding a core of Glucagon (A) and Insulin (B) producing cells; however frequent clusters of D cells are intermingled with the other endocrine cells. Statistical evaluations on the percentages of these 3 cell types showed preponderance of A cells in Natrix whereas in Vipera no significant difference was found between the number of A and B cells. The D cells showed a uniform distribution in the pancreas of the 2 studied species, in any case with a percentage slightly inferior to those of B cells.